Dr. Sunil Kumar Singh

FACULTY OF SCIENCE >> Department of Botany
Designation : Professor
Qualification : -MSc: University of Lucknow, Lucknow, India. -PhD: National Botanical Research Institute, Lucknow, India.
Phone Nos : 00919737515440
Email :
Experience : -Postdoctoral Researcher: Institute of Plant Sciences, -Postdoctoral Researcher: Institute of Plant Sciences, ARO, Israel. -Postdoctoral Researcher: Umea Plant Science Centre, Umea, Sweden. -Assistant Professor & Group Leader: Indian Institute of Advanced Research (IIAR), Gandhinagar, India. -Associate Professor: Department of Botany, Faculty of Science, The MS University of Baroda, Vadodara, India.
Areas of Interest : -Developmental and stress induced cell death in plants. -Cell wall and vascular tissues development. -Plant Functional Genomics. -Genetic improvement of plants.
Dr. Sunil Kumar Singh

Detailed CV

Dr. Sunil Kumar Singh
Associate Professor
Plant Cell and Molecular Biology Lab
Department of Botany
Faculty of Science
The MS University of Baroda
Vadodara-390 002, Gujarat, India
Email: sunilkrsingh@hotmail.com  
Phone (M): +91-(0)9737515440
Education and Professional Experience
-   MSc: University of Lucknow, Lucknow, India.
-   PhD: National Botanical Research Institute, Lucknow, India.
-   Postdoctoral Researcher: Institute of Plant Sciences, ARO, Israel.
-   Postdoctoral Researcher: Umea Plant Science Centre, Umea, Sweden.
-   Assistant Professor & Group Leader: Indian Institute of Advanced Research (IIAR), Gandhinagar, India.
-   Associate Professor: Department of Botany, Faculty of Science, The MS University of Baroda, Vadodara, India.
-   Postdoctoral Fellowship (MASHAV) at Institute of Plant Sciences, Agricultural Research Organization, Israel, 2001-2003.
-   Postdoctoral Fellowships at Umea Plant Science Centre from Formas and Swedish Research Council, Sweden, 2003-2009.
-   Member of EPSO European Plant Science Organization-2009-2010.
Research Interests
-   Developmental and stress induced cell death in plants.
-   Cell wall and vascular tissues development.
-   Plant Functional Genomics.
-   Genetic improvement of plants.
Ongoing Research Work
-   Developmental and stress related role of Metacaspases in Arabidopsis thaliana and Solanum lycopersicum.
-   Role of some Polyamine biosynthetic genes during plant senescence and stress responses in Solanum lycopersicum.
-   Understanding the role of some Ubiquitin-Conjugase Enzymes and GARP-G2 like transcriptional factors in Arabidopsis thaliana.
Description of Research Work
Programmed cell death plays important role during plant development. Cell death can also occur due to molecular damage caused by ROS molecules and various environemtal stresses. The developmental cell death and environmently induced cell death are linked, as both shares many common morphological, biochemical and genetical features. There are many cell death and related genes which may be interesting candidates as they are shown to upregulated in cells and tissues undergoing cell death during plant development and stresses. We are mainly interested to know how developmental and environment induced cell death is regulated in plants.
We are also interested to understand  the regulatory mechanism that control differentiation of plant vascular tissues. Vascular tissues are composed of very specialized cells and their differentiation is very complex process. We are aiming to characterize the role of some novel genes that are involved in differentiation of  these tissues.
Using various genomic and bioinformatic resources, we have identified some interesting candidate genes which includes, Caspase-like-genes, genes involved in ubiquitination, polyamine biosynthetic genes, MYB and GARP-G2 like transcriptional factors. We are using molecular genetic, cell biological and biochemical based approaches to characterize their role in Arabidopsis, tomato and tobacco. Gene expression analysis, RNAi, CRISPR/Cas9-genome editing system, gene overexpression, mutant analysis, cell and tissues specific markers, in-situ localisation, and biochemical assays are being used to determine their developmental as well as stress related role in plants.
Selected Publications
-   Rosenbluh J, Singh SK, Gafni Y, Graessmann A and Loyter A. 2004. Non-endocytic penetration of core histones into petunia protoplasts and cultured cells: a novel mechanism for the introduction of macromolecules into plant cells. Biochemica et Biophysica Acta.1664: 230-240.
-   Singh SK, Eland C, Harholt J, Scheller HV and Marchant A. 2005. Cell adhesion in Arabidopsis thaliana is mediated by ECTOPICALLY PARTING CELLS 1-a glycosyltransferase (GT64) related to the animal exostosins. The Plant Journal. 43: 384-397.
-   *Dharmasiri S, *Swarup R, *Mockaitis K, Dharmasiri N, Singh SK, Kowalchyk M, Marchant A, Mills S, Sandberg G, Bennett MJ, and Estelle M. 2006. AXR4 is required for asymmetric localization of the auxin influx facilitator AUX1. Science. 312: 1218-1220 (Article received recognition by Faculty of 1000).
-   Ubeda-Tomas S, Edvardsson E, Eland C, Singh SK, Zadik D, Aspeborg H, Gorzsas A, Teeri TT, Sundberg B., Persson P, Bennett M and Marchant A. 2007. Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues. Physiologia plantarum. 129: 415-428.
-   Singh SK, Fischer U, Singh M, Grebe M and Marchant A. 2008. Insight into the early steps of root hair formation revealed by the procuste1 cellulose synthase mutant of Arabidopsis thaliana. BMC Plant Biology. 8: 57(1-12).
-   *Muniz L, *Minguet EG, *Singh SK, Pesquet E, Vera-Sirera F, Courtois-Moreau C, Carbonell J, Blazquez MA, and Tuominen H. 2008. ACAULIS 5 controls Arabidopsis xylem specification through the prevention of premature cell death. Development. 135: 2573-2582 (Article chosen for journal cover page).
-   Wu A-M, Rihouey C, Seveno M, Hörnblad E, Singh SK, Matsunaga T, Ishii T, Lerouge P and Marchant A. 2009. The Arabidopsis IRX10 and IRX10-LIKE glycosyltransferases are critical for glucuronoxylan biosynthesis during secondary cell wall formation. The Plant Journal 57: 718-731.
-   Vera-Sirera F, Minguet FG, Singh SK, Ljung K, Tuominen H, Blázquez MA and Carbonell J. 2010. Role of polyamines in plant vascular development. Plant Physiology and Biochemistry 48: 534-539.
-   Edvardsson E, Singh SK, Yun M-S, Mansfeld A, Hauser M-T and Marchant A. 2011. The Plant Glycosyltransferase Family GT64: In Search of a Function. Annual Plant Reviews: Plant Polysaccharides, Biosynthesis and Bioengineering. 41: 285-304.
-   Ouyang K, Li J, Zhao X, Que Q, Li P, Huang H, Que Q, Li P, Huang H, Deng H, Singh SK, Wu A-M, Chen X (2016).Transcriptomic analysis of multipurpose timber yielding tree Neolamarckia cadamba during xylogenesis using RNA-Seq. PLoS ONE 11(7): e0159407.
Courses being taught during 2016-17
-   IDS1102 Foundation Course in Biology-I
-   BOT1112, BOT1115 Plant Kingdom-II
-   BOT1117 Introductory Botany-I
-   BOT1202, BOT1205 Cell Biology
-   BOT1315 Basic Microbiology and Plant Physiology                                                                                   
-   BOT1412, BOT1415 Economic Biology
-   BOT1418 Horticulture and Economic Botany
-   BOT1603 Genetics and Molecular Biology
-   CMB1603 Developmental Biology
-   CMB1704 Plant Physiology
-   BOT2203 Basic Molecular Biology
Workshops/ Meeting/ Programme  Organized
-   Organized Sixth Meeting of Programme Advisory Committee (PAC) on Plant Sciences, SERB, New Delhi at The MS University of Baroda, Vadodara-390 002 on 13-14 February, 2014.
-   Organized a workshop on "Writing Research Proposals" at Indian Institute of Advanced Research (IIAR), Gandhinagar-382007 on 3-4, February, 2011 with the support of GSBTM, Gujarat.
-   Coordinated M.Sc. programme in Plant Molecular Biology and Biotechnology, 2010 - 2013 at IIAR.
Working JRF and Doctoral Students
-   Manisha Farsodia, DST-Inspire SRF/doctoral student,  Understanding the role of Polyamine biosynthetic genes in tomato.
-   Vishal Vyas, DBT-JRF/doctoral student, Characterizing the role of AtMC3 in Arabidopsis.
-   Maitri Trivedi, SERB-JRF/doctoral student, Role of Type II Metcaspases in tomato.
-   Gouri Pandya, SERB-JRF, Role of Type I Metacaspases in tomato.


26-11-2016 16:38:27